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c2768a cell lines  (Addgene inc)


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    Addgene inc c2768a cell lines
    FIGURE 2 Inducible Ubr5 expression knockdown suppresses post-surgery lung metastasis. (A) Schematic for removing primary tumor and knocking down Ubr5 expression with daily Dox addition (100 mg/kg) by gavage post-surgery in mice. (B, C) Representative images for lung nodules stained with India ink (B) Lung nodules in tumor-bearing are quantified (C). (D, E) The 6-thioguanine clonogenicity assay of Tet-scr and Tet-shUbr5 tumors (on D35) for lung metastasis is conducted. Images of representative experiment are depicted (D) and colonies are quantified (E). (F) Overall survival rates of the mice (Tet-scr, n = 8; Tet-scr(+Dox), n = 6; Tet-shUbr5, n = 5; Tet-shUbr5(+Dox), n = 5). (G) UBR5 protein expression in hUBR5- and <t>C2768A-reconstituted</t> 4T1/Ubr5/ cells is evaluated in Western blotting. (H) Representative result of E2 discharge assay for UBR5 and C2768A. (I) Quantitation of the discharged E2-Ub by UBR5 and C2768A. (J) A total of 5 105 Tet-scr, Tet-shUbr5, Ubr5/ + hUBR5 or Ubr5/ + C2768A cells are injected into 6- to 8-week-old female BALB/c mice (with 8, 8, 8 and 16 mice, respectively, for the four cell lines). Tumor volume is measured on D20. (K) The metastatic cells in the lung are enumerated by the 6-thiogaunine assay. (L) After removing the primary tumor, the mice with similar primary tumor volumes are selected from all groups and administrated with Dox daily by gavage (100 mg/kg). On D30, the mice are sacrificed. Lung metastatic nodules are stained with Indian ink and quantified (Tet-scr, n = 5; Tet-shUbr5, n = 5; Ubr5/ + hUBR5, n = 5, Ubr5/ + C2768A, n = 4). Data represents mean ± SEM; *P < .05; **P < .01; ***P < .001. [Color figure can be viewed at wileyonlinelibrary.com]
    C2768a Cell Lines, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c2768a+cell+lines/pm37855385-36-9-18?v=Addgene+inc
    Average 93 stars, based on 29 article reviews
    c2768a cell lines - by Bioz Stars, 2026-08
    93/100 stars

    Images

    1) Product Images from "Targeting UBR5 inhibits postsurgical breast cancer lung metastases by inducing CDC73 and p53 mediated apoptosis."

    Article Title: Targeting UBR5 inhibits postsurgical breast cancer lung metastases by inducing CDC73 and p53 mediated apoptosis.

    Journal: International journal of cancer

    doi: 10.1002/ijc.34769

    FIGURE 2 Inducible Ubr5 expression knockdown suppresses post-surgery lung metastasis. (A) Schematic for removing primary tumor and knocking down Ubr5 expression with daily Dox addition (100 mg/kg) by gavage post-surgery in mice. (B, C) Representative images for lung nodules stained with India ink (B) Lung nodules in tumor-bearing are quantified (C). (D, E) The 6-thioguanine clonogenicity assay of Tet-scr and Tet-shUbr5 tumors (on D35) for lung metastasis is conducted. Images of representative experiment are depicted (D) and colonies are quantified (E). (F) Overall survival rates of the mice (Tet-scr, n = 8; Tet-scr(+Dox), n = 6; Tet-shUbr5, n = 5; Tet-shUbr5(+Dox), n = 5). (G) UBR5 protein expression in hUBR5- and C2768A-reconstituted 4T1/Ubr5/ cells is evaluated in Western blotting. (H) Representative result of E2 discharge assay for UBR5 and C2768A. (I) Quantitation of the discharged E2-Ub by UBR5 and C2768A. (J) A total of 5 105 Tet-scr, Tet-shUbr5, Ubr5/ + hUBR5 or Ubr5/ + C2768A cells are injected into 6- to 8-week-old female BALB/c mice (with 8, 8, 8 and 16 mice, respectively, for the four cell lines). Tumor volume is measured on D20. (K) The metastatic cells in the lung are enumerated by the 6-thiogaunine assay. (L) After removing the primary tumor, the mice with similar primary tumor volumes are selected from all groups and administrated with Dox daily by gavage (100 mg/kg). On D30, the mice are sacrificed. Lung metastatic nodules are stained with Indian ink and quantified (Tet-scr, n = 5; Tet-shUbr5, n = 5; Ubr5/ + hUBR5, n = 5, Ubr5/ + C2768A, n = 4). Data represents mean ± SEM; *P < .05; **P < .01; ***P < .001. [Color figure can be viewed at wileyonlinelibrary.com]
    Figure Legend Snippet: FIGURE 2 Inducible Ubr5 expression knockdown suppresses post-surgery lung metastasis. (A) Schematic for removing primary tumor and knocking down Ubr5 expression with daily Dox addition (100 mg/kg) by gavage post-surgery in mice. (B, C) Representative images for lung nodules stained with India ink (B) Lung nodules in tumor-bearing are quantified (C). (D, E) The 6-thioguanine clonogenicity assay of Tet-scr and Tet-shUbr5 tumors (on D35) for lung metastasis is conducted. Images of representative experiment are depicted (D) and colonies are quantified (E). (F) Overall survival rates of the mice (Tet-scr, n = 8; Tet-scr(+Dox), n = 6; Tet-shUbr5, n = 5; Tet-shUbr5(+Dox), n = 5). (G) UBR5 protein expression in hUBR5- and C2768A-reconstituted 4T1/Ubr5/ cells is evaluated in Western blotting. (H) Representative result of E2 discharge assay for UBR5 and C2768A. (I) Quantitation of the discharged E2-Ub by UBR5 and C2768A. (J) A total of 5 105 Tet-scr, Tet-shUbr5, Ubr5/ + hUBR5 or Ubr5/ + C2768A cells are injected into 6- to 8-week-old female BALB/c mice (with 8, 8, 8 and 16 mice, respectively, for the four cell lines). Tumor volume is measured on D20. (K) The metastatic cells in the lung are enumerated by the 6-thiogaunine assay. (L) After removing the primary tumor, the mice with similar primary tumor volumes are selected from all groups and administrated with Dox daily by gavage (100 mg/kg). On D30, the mice are sacrificed. Lung metastatic nodules are stained with Indian ink and quantified (Tet-scr, n = 5; Tet-shUbr5, n = 5; Ubr5/ + hUBR5, n = 5, Ubr5/ + C2768A, n = 4). Data represents mean ± SEM; *P < .05; **P < .01; ***P < .001. [Color figure can be viewed at wileyonlinelibrary.com]

    Techniques Used: Expressing, Knockdown, Staining, Western Blot, Quantitation Assay, Injection



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    c2768a cell lines  (Addgene inc)
    93
    Addgene inc c2768a cell lines
    FIGURE 2 Inducible Ubr5 expression knockdown suppresses post-surgery lung metastasis. (A) Schematic for removing primary tumor and knocking down Ubr5 expression with daily Dox addition (100 mg/kg) by gavage post-surgery in mice. (B, C) Representative images for lung nodules stained with India ink (B) Lung nodules in tumor-bearing are quantified (C). (D, E) The 6-thioguanine clonogenicity assay of Tet-scr and Tet-shUbr5 tumors (on D35) for lung metastasis is conducted. Images of representative experiment are depicted (D) and colonies are quantified (E). (F) Overall survival rates of the mice (Tet-scr, n = 8; Tet-scr(+Dox), n = 6; Tet-shUbr5, n = 5; Tet-shUbr5(+Dox), n = 5). (G) UBR5 protein expression in hUBR5- and <t>C2768A-reconstituted</t> 4T1/Ubr5/ cells is evaluated in Western blotting. (H) Representative result of E2 discharge assay for UBR5 and C2768A. (I) Quantitation of the discharged E2-Ub by UBR5 and C2768A. (J) A total of 5 105 Tet-scr, Tet-shUbr5, Ubr5/ + hUBR5 or Ubr5/ + C2768A cells are injected into 6- to 8-week-old female BALB/c mice (with 8, 8, 8 and 16 mice, respectively, for the four cell lines). Tumor volume is measured on D20. (K) The metastatic cells in the lung are enumerated by the 6-thiogaunine assay. (L) After removing the primary tumor, the mice with similar primary tumor volumes are selected from all groups and administrated with Dox daily by gavage (100 mg/kg). On D30, the mice are sacrificed. Lung metastatic nodules are stained with Indian ink and quantified (Tet-scr, n = 5; Tet-shUbr5, n = 5; Ubr5/ + hUBR5, n = 5, Ubr5/ + C2768A, n = 4). Data represents mean ± SEM; *P < .05; **P < .01; ***P < .001. [Color figure can be viewed at wileyonlinelibrary.com]
    C2768a Cell Lines, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c2768a+cell+lines/pm37855385-36-9-18?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    c2768a cell lines - by Bioz Stars, 2026-08
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    FIGURE 2 Inducible Ubr5 expression knockdown suppresses post-surgery lung metastasis. (A) Schematic for removing primary tumor and knocking down Ubr5 expression with daily Dox addition (100 mg/kg) by gavage post-surgery in mice. (B, C) Representative images for lung nodules stained with India ink (B) Lung nodules in tumor-bearing are quantified (C). (D, E) The 6-thioguanine clonogenicity assay of Tet-scr and Tet-shUbr5 tumors (on D35) for lung metastasis is conducted. Images of representative experiment are depicted (D) and colonies are quantified (E). (F) Overall survival rates of the mice (Tet-scr, n = 8; Tet-scr(+Dox), n = 6; Tet-shUbr5, n = 5; Tet-shUbr5(+Dox), n = 5). (G) UBR5 protein expression in hUBR5- and C2768A-reconstituted 4T1/Ubr5/ cells is evaluated in Western blotting. (H) Representative result of E2 discharge assay for UBR5 and C2768A. (I) Quantitation of the discharged E2-Ub by UBR5 and C2768A. (J) A total of 5 105 Tet-scr, Tet-shUbr5, Ubr5/ + hUBR5 or Ubr5/ + C2768A cells are injected into 6- to 8-week-old female BALB/c mice (with 8, 8, 8 and 16 mice, respectively, for the four cell lines). Tumor volume is measured on D20. (K) The metastatic cells in the lung are enumerated by the 6-thiogaunine assay. (L) After removing the primary tumor, the mice with similar primary tumor volumes are selected from all groups and administrated with Dox daily by gavage (100 mg/kg). On D30, the mice are sacrificed. Lung metastatic nodules are stained with Indian ink and quantified (Tet-scr, n = 5; Tet-shUbr5, n = 5; Ubr5/ + hUBR5, n = 5, Ubr5/ + C2768A, n = 4). Data represents mean ± SEM; *P < .05; **P < .01; ***P < .001. [Color figure can be viewed at wileyonlinelibrary.com]

    Journal: International journal of cancer

    Article Title: Targeting UBR5 inhibits postsurgical breast cancer lung metastases by inducing CDC73 and p53 mediated apoptosis.

    doi: 10.1002/ijc.34769

    Figure Lengend Snippet: FIGURE 2 Inducible Ubr5 expression knockdown suppresses post-surgery lung metastasis. (A) Schematic for removing primary tumor and knocking down Ubr5 expression with daily Dox addition (100 mg/kg) by gavage post-surgery in mice. (B, C) Representative images for lung nodules stained with India ink (B) Lung nodules in tumor-bearing are quantified (C). (D, E) The 6-thioguanine clonogenicity assay of Tet-scr and Tet-shUbr5 tumors (on D35) for lung metastasis is conducted. Images of representative experiment are depicted (D) and colonies are quantified (E). (F) Overall survival rates of the mice (Tet-scr, n = 8; Tet-scr(+Dox), n = 6; Tet-shUbr5, n = 5; Tet-shUbr5(+Dox), n = 5). (G) UBR5 protein expression in hUBR5- and C2768A-reconstituted 4T1/Ubr5/ cells is evaluated in Western blotting. (H) Representative result of E2 discharge assay for UBR5 and C2768A. (I) Quantitation of the discharged E2-Ub by UBR5 and C2768A. (J) A total of 5 105 Tet-scr, Tet-shUbr5, Ubr5/ + hUBR5 or Ubr5/ + C2768A cells are injected into 6- to 8-week-old female BALB/c mice (with 8, 8, 8 and 16 mice, respectively, for the four cell lines). Tumor volume is measured on D20. (K) The metastatic cells in the lung are enumerated by the 6-thiogaunine assay. (L) After removing the primary tumor, the mice with similar primary tumor volumes are selected from all groups and administrated with Dox daily by gavage (100 mg/kg). On D30, the mice are sacrificed. Lung metastatic nodules are stained with Indian ink and quantified (Tet-scr, n = 5; Tet-shUbr5, n = 5; Ubr5/ + hUBR5, n = 5, Ubr5/ + C2768A, n = 4). Data represents mean ± SEM; *P < .05; **P < .01; ***P < .001. [Color figure can be viewed at wileyonlinelibrary.com]

    Article Snippet: To generate 4T1/Ubr5 / + hUBR5 and 4T1/Ubr5 / + C2768A cell lines, pCMV-Tag2B UBR5 and pCMV-Tag2B C2768A (Addgene, #37188 and #37189, respectively) were transfected into 4T1/Ubr5 / cells using Lipofectamine 3000 reagent (L3000008, Invitrogen, Waltham, MA).

    Techniques: Expressing, Knockdown, Staining, Western Blot, Quantitation Assay, Injection